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antibodies against mouse pi3k  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc antibodies against mouse pi3k
(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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primary rabbit anti mouse pi3k  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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primary rabbit anti mouse p pi3k  (Danaher Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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sc 133 rrid ab 632305 rabbit polyclonal anti pi3k p110α cell signaling 4255 rrid ab 659888 mouse monoclonal anti rassf7  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
Sc 133 Rrid Ab 632305 Rabbit Polyclonal Anti Pi3k P110α Cell Signaling 4255 Rrid Ab 659888 Mouse Monoclonal Anti Rassf7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti pi3k  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
Rabbit Anti Pi3k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti rabbit phospho-pi3k (phospho-4249)  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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tbst diluted rabbit anti rat primary antibodies pi3k  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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anti-p-pi3k rabbit anti-human/mouse antibody  (EnoGene Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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wanleibio 4 pi3k rabbit  (Cell Signaling Technology Inc)
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(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of <t>PI3K,</t> p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.
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Image Search Results


(A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.

Journal: PLOS Pathogens

Article Title: Interleukin-17A signaling promotes CD8 + T cell cytotoxicity against West Nile virus infection through enhancing PI3K-mTOR-mediated metabolism

doi: 10.1371/journal.ppat.1013218

Figure Lengend Snippet: (A) Eight weeks old Il17ra -/- , and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8 + T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. (B-D) Eight to nine weeks old Il17a -/- (n = 5), Il17rc -/- (n = 4), and WT (n = 8) mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, cultured ex vivo with mouse rIL-17A (50 ng/ml) or RPMI (control) for 24 h and measured the gene expression of PIK3ca (B), mTOR (C), and S6K1 (D) by RT-qPCR, expressed as the relative fold changes (RFC). Data were analyzed by two-tailed student’s t- tests (B-D) and presented as mean ± s.e.m. with *, and ** denoting p <0.05, and p <0.01, respectively. (E-J) Eight to nine weeks-old WT mice were infected with 20 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i. CD8 + T cells were isolated from the splenocytes, stimulated ex vivo with mouse rIL-17A (100 ng/ml) or RPMI (control), and co-treated with either rapamycin (20 nM), LY294002 (12.5 µM) for 15- or 60- minutes. Cell lysates were resolved with SDS-PAGE. The protein expressions of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, p -mTOR Ser2481 , pS6K, p -pS6K Thr389 , 4EBP1, and p-4EBP1 Thr37/46 were analyzed by immunoblot. GAPDH was used as the loading control. (E) The protein bands of PI3K, p -PI3K Tyr458 , AKT, p -AKT Thr308 , mTOR, and p -mTOR Ser2481 were from the 15-min treatment, and the remaining bands were from the 60-min treatment. (F-J) Densitometric quantification of the phosphorylated protein normalized to corresponding total protein and analyzed by Image Lab software.

Article Snippet: The proteins were then transferred into a nitrocellulose membrane and probed with primary antibodies against mouse PI3K (p85, 1:500, Cell Signaling Technology, 4257), phosphorated ( p )-PI3K (p85 [Tyr458]/p55 [Tyr199], 1:200, Cell Signaling Technology, 4228), AKT (1:500, Cell Signaling Technology, 9272), p -AKT (Thr308, 1:200, Cell Signaling Technology, 4056), mTOR (1:500, Cell Signaling Technology, 2972), p -mTOR (Ser2481, 1:200, Cell Signaling Technology, 2974), pS6K (1:500, Cell Signaling Technology, 9202), p -pS6K (Thr389, 1:200, Cell Signaling Technology, 9234), 4EBP1 (1:500, Cell Signaling Technology, 9644), p -4EBP1 (Thr37/46, 1:500, Cell Signaling Technology, 2855), and GAPDH as control (1:1000, Cell Signaling Technology, 5174) and incubated overnight at 4°C.

Techniques: Infection, Purification, RNA Sequencing, Isolation, Cell Culture, Ex Vivo, Control, Gene Expression, Quantitative RT-PCR, Two Tailed Test, SDS Page, Western Blot, Software